Western Blot:Article Title: Elevated FBXO45 promotes TFG ubiquitination and drives lung metastasis of hepatocellular carcinoma
Article Snippet: Antibodies Source Identifiers Additional information Mouse anti-Flag Sigma Cat# F1804; RRID: AB_262044 WB (1:1000) Rat anti-HA Roche Cat# 11867423001; RRID: AB_390918 WB (1:1000) Rabbit anti-FBXO45 Bioss Cat# bs-13150R WB (1:1000) IHC (1:300) Rabbit anti-TFG Proteintech Cat# 11571-1-AP; RRID: AB_2203102 WB (1:2000) IHC (1:200) Mouse anti-β-actin Proteintech Cat# 20536-1-AP; RRID: AB_10700003 WB (1:5000) Rabbit anti-GAPDH Proteintech Cat# 10494-1-AP; RRID: AB_2263076 WB (1:5000) Rabbit anti-vimentin Proteintech Cat# 10366-1-AP; RRID: AB_2273020 WB (1:5000) Rabbit anti-LIPC Proteintech Cat# 21133-1-AP; RRID: AB_11041715 IHC (1:200) Rabbit anti-TP53 Proteintech Cat# 10442-1-AP; RRID: AB_2206609 IHC (1:300) Rabbit anti-SKP1 Proteintech Cat# 10990-2-AP; RRID: AB_2187492 WB (1:1000) Rabbit anti-PAM Proteintech Cat# 26972-1-AP; RRID: AB_2880706 WB (1:3000) Rabbit anti-ATF2 Proteintech Cat# 14834-1-AP; RRID: AB_10694139 WB (1:1000) Goat anti-mouse IgG HRP Cell Signaling Cat# 7076S; RRID: AB_330924 WB (1:5000) Goat anti-rabbit IgG HRP Cell Signaling Cat# 7074S; RRID: AB_2099233 WB (1:5000) Goat anti-rat IgG HRP Cell Signaling Cat# 7077S; RRID: AB_10694715 WB (1:5000) Mouse anti-N-cadherin Cell Signaling Cat# 14215S; RRID: AB_2798427 WB (1:1000) Mouse anti-E-cadherin Cell Signaling Cat# 14472S; RRID: AB_2728770 WB (1:1000) Rabbit anti-snail Cell Signaling Cat# 3879S; RRID: AB_2255011 WB (1:1000) Rabbit anti-NF-κB p65 Cell Signaling Cat# 8242S; RRID: AB_10859369 WB (1:1000) Mouse IgG1 Isotype Control Cell Signaling Cat# 5415S; 25 RRID: AB_10829607 Rabbit IgG1 Isotype Control Cell Signaling Cat# 3900S; RRID: AB_1550038
Article Title: Elevated FBXO45 promotes TFG ubiquitination and drives lung metastasis of hepatocellular carcinoma
Article Snippet: Antibodies Source Identifiers Additional information Mouse anti-Flag Sigma Cat# F1804; RRID: AB_262044 WB (1:1000) Rat anti-HA Roche Cat# 11867423001; RRID: AB_390918 WB (1:1000) Rabbit anti-FBXO45 Bioss Cat# bs-13150R WB (1:1000) IHC (1:300) Rabbit anti-TFG Proteintech Cat# 11571-1-AP; RRID: AB_2203102 WB (1:2000) IHC (1:200) Mouse anti-β-actin Proteintech Cat# 20536-1-AP; RRID: AB_10700003 WB (1:5000) Rabbit anti-GAPDH Proteintech Cat# 10494-1-AP; RRID: AB_2263076 WB (1:5000) Rabbit anti-vimentin Proteintech Cat# 10366-1-AP; RRID: AB_2273020 WB (1:5000) Rabbit anti-LIPC Proteintech Cat# 21133-1-AP; RRID: AB_11041715 IHC (1:200) Rabbit anti-TP53 Proteintech Cat# 10442-1-AP; RRID: AB_2206609 IHC (1:300) Rabbit anti-SKP1 Proteintech Cat# 10990-2-AP; RRID: AB_2187492 WB (1:1000) Rabbit anti-PAM Proteintech Cat# 26972-1-AP; RRID: AB_2880706 WB (1:3000) Rabbit anti-ATF2 Proteintech Cat# 14834-1-AP; RRID: AB_10694139 WB (1:1000) Goat anti-mouse IgG HRP Cell Signaling Cat# 7076S; RRID: AB_330924 WB (1:5000) Goat anti-rabbit IgG HRP Cell Signaling Cat# 7074S; RRID: AB_2099233 WB (1:5000) Goat anti-rat IgG HRP Cell Signaling Cat# 7077S; RRID: AB_10694715 WB (1:5000) Mouse anti-N-cadherin Cell Signaling Cat# 14215S; RRID: AB_2798427 WB (1:1000) Mouse anti-E-cadherin Cell Signaling Cat# 14472S; RRID: AB_2728770 WB (1:1000) Rabbit anti-snail Cell Signaling Cat# 3879S; RRID: AB_2255011 WB (1:1000) Rabbit anti-NF-κB p65 Cell Signaling Cat# 8242S; RRID: AB_10859369 WB (1:1000) Mouse IgG1 Isotype Control Cell Signaling Cat# 5415S; RRID: AB_10829607 Rabbit IgG1 Isotype Control Cell Signaling Cat# 3900S; RRID: AB_1550038
Immunohistochemistry:Article Title: Elevated FBXO45 promotes TFG ubiquitination and drives lung metastasis of hepatocellular carcinoma
Article Snippet: Antibodies Source Identifiers Additional information Mouse anti-Flag Sigma Cat# F1804; RRID: AB_262044 WB (1:1000) Rat anti-HA Roche Cat# 11867423001; RRID: AB_390918 WB (1:1000) Rabbit anti-FBXO45 Bioss Cat# bs-13150R WB (1:1000) IHC (1:300) Rabbit anti-TFG Proteintech Cat# 11571-1-AP; RRID: AB_2203102 WB (1:2000) IHC (1:200) Mouse anti-β-actin Proteintech Cat# 20536-1-AP; RRID: AB_10700003 WB (1:5000) Rabbit anti-GAPDH Proteintech Cat# 10494-1-AP; RRID: AB_2263076 WB (1:5000) Rabbit anti-vimentin Proteintech Cat# 10366-1-AP; RRID: AB_2273020 WB (1:5000) Rabbit anti-LIPC Proteintech Cat# 21133-1-AP; RRID: AB_11041715 IHC (1:200) Rabbit anti-TP53 Proteintech Cat# 10442-1-AP; RRID: AB_2206609 IHC (1:300) Rabbit anti-SKP1 Proteintech Cat# 10990-2-AP; RRID: AB_2187492 WB (1:1000) Rabbit anti-PAM Proteintech Cat# 26972-1-AP; RRID: AB_2880706 WB (1:3000) Rabbit anti-ATF2 Proteintech Cat# 14834-1-AP; RRID: AB_10694139 WB (1:1000) Goat anti-mouse IgG HRP Cell Signaling Cat# 7076S; RRID: AB_330924 WB (1:5000) Goat anti-rabbit IgG HRP Cell Signaling Cat# 7074S; RRID: AB_2099233 WB (1:5000) Goat anti-rat IgG HRP Cell Signaling Cat# 7077S; RRID: AB_10694715 WB (1:5000) Mouse anti-N-cadherin Cell Signaling Cat# 14215S; RRID: AB_2798427 WB (1:1000) Mouse anti-E-cadherin Cell Signaling Cat# 14472S; RRID: AB_2728770 WB (1:1000) Rabbit anti-snail Cell Signaling Cat# 3879S; RRID: AB_2255011 WB (1:1000) Rabbit anti-NF-κB p65 Cell Signaling Cat# 8242S; RRID: AB_10859369 WB (1:1000) Mouse IgG1 Isotype Control Cell Signaling Cat# 5415S; 25 RRID: AB_10829607 Rabbit IgG1 Isotype Control Cell Signaling Cat# 3900S; RRID: AB_1550038
Article Title: Elevated FBXO45 promotes TFG ubiquitination and drives lung metastasis of hepatocellular carcinoma
Article Snippet: Antibodies Source Identifiers Additional information Mouse anti-Flag Sigma Cat# F1804; RRID: AB_262044 WB (1:1000) Rat anti-HA Roche Cat# 11867423001; RRID: AB_390918 WB (1:1000) Rabbit anti-FBXO45 Bioss Cat# bs-13150R WB (1:1000) IHC (1:300) Rabbit anti-TFG Proteintech Cat# 11571-1-AP; RRID: AB_2203102 WB (1:2000) IHC (1:200) Mouse anti-β-actin Proteintech Cat# 20536-1-AP; RRID: AB_10700003 WB (1:5000) Rabbit anti-GAPDH Proteintech Cat# 10494-1-AP; RRID: AB_2263076 WB (1:5000) Rabbit anti-vimentin Proteintech Cat# 10366-1-AP; RRID: AB_2273020 WB (1:5000) Rabbit anti-LIPC Proteintech Cat# 21133-1-AP; RRID: AB_11041715 IHC (1:200) Rabbit anti-TP53 Proteintech Cat# 10442-1-AP; RRID: AB_2206609 IHC (1:300) Rabbit anti-SKP1 Proteintech Cat# 10990-2-AP; RRID: AB_2187492 WB (1:1000) Rabbit anti-PAM Proteintech Cat# 26972-1-AP; RRID: AB_2880706 WB (1:3000) Rabbit anti-ATF2 Proteintech Cat# 14834-1-AP; RRID: AB_10694139 WB (1:1000) Goat anti-mouse IgG HRP Cell Signaling Cat# 7076S; RRID: AB_330924 WB (1:5000) Goat anti-rabbit IgG HRP Cell Signaling Cat# 7074S; RRID: AB_2099233 WB (1:5000) Goat anti-rat IgG HRP Cell Signaling Cat# 7077S; RRID: AB_10694715 WB (1:5000) Mouse anti-N-cadherin Cell Signaling Cat# 14215S; RRID: AB_2798427 WB (1:1000) Mouse anti-E-cadherin Cell Signaling Cat# 14472S; RRID: AB_2728770 WB (1:1000) Rabbit anti-snail Cell Signaling Cat# 3879S; RRID: AB_2255011 WB (1:1000) Rabbit anti-NF-κB p65 Cell Signaling Cat# 8242S; RRID: AB_10859369 WB (1:1000) Mouse IgG1 Isotype Control Cell Signaling Cat# 5415S; RRID: AB_10829607 Rabbit IgG1 Isotype Control Cell Signaling Cat# 3900S; RRID: AB_1550038
Control:Article Title: Elevated FBXO45 promotes TFG ubiquitination and drives lung metastasis of hepatocellular carcinoma
Article Snippet: Antibodies Source Identifiers Additional information Mouse anti-Flag Sigma Cat# F1804; RRID: AB_262044 WB (1:1000) Rat anti-HA Roche Cat# 11867423001; RRID: AB_390918 WB (1:1000) Rabbit anti-FBXO45 Bioss Cat# bs-13150R WB (1:1000) IHC (1:300) Rabbit anti-TFG Proteintech Cat# 11571-1-AP; RRID: AB_2203102 WB (1:2000) IHC (1:200) Mouse anti-β-actin Proteintech Cat# 20536-1-AP; RRID: AB_10700003 WB (1:5000) Rabbit anti-GAPDH Proteintech Cat# 10494-1-AP; RRID: AB_2263076 WB (1:5000) Rabbit anti-vimentin Proteintech Cat# 10366-1-AP; RRID: AB_2273020 WB (1:5000) Rabbit anti-LIPC Proteintech Cat# 21133-1-AP; RRID: AB_11041715 IHC (1:200) Rabbit anti-TP53 Proteintech Cat# 10442-1-AP; RRID: AB_2206609 IHC (1:300) Rabbit anti-SKP1 Proteintech Cat# 10990-2-AP; RRID: AB_2187492 WB (1:1000) Rabbit anti-PAM Proteintech Cat# 26972-1-AP; RRID: AB_2880706 WB (1:3000) Rabbit anti-ATF2 Proteintech Cat# 14834-1-AP; RRID: AB_10694139 WB (1:1000) Goat anti-mouse IgG HRP Cell Signaling Cat# 7076S; RRID: AB_330924 WB (1:5000) Goat anti-rabbit IgG HRP Cell Signaling Cat# 7074S; RRID: AB_2099233 WB (1:5000) Goat anti-rat IgG HRP Cell Signaling Cat# 7077S; RRID: AB_10694715 WB (1:5000) Mouse anti-N-cadherin Cell Signaling Cat# 14215S; RRID: AB_2798427 WB (1:1000) Mouse anti-E-cadherin Cell Signaling Cat# 14472S; RRID: AB_2728770 WB (1:1000) Rabbit anti-snail Cell Signaling Cat# 3879S; RRID: AB_2255011 WB (1:1000) Rabbit anti-NF-κB p65 Cell Signaling Cat# 8242S; RRID: AB_10859369 WB (1:1000) Mouse IgG1 Isotype Control Cell Signaling Cat# 5415S; 25 RRID: AB_10829607 Rabbit IgG1 Isotype Control Cell Signaling Cat# 3900S; RRID: AB_1550038
Article Title: Elevated FBXO45 promotes TFG ubiquitination and drives lung metastasis of hepatocellular carcinoma
Article Snippet: Antibodies Source Identifiers Additional information Mouse anti-Flag Sigma Cat# F1804; RRID: AB_262044 WB (1:1000) Rat anti-HA Roche Cat# 11867423001; RRID: AB_390918 WB (1:1000) Rabbit anti-FBXO45 Bioss Cat# bs-13150R WB (1:1000) IHC (1:300) Rabbit anti-TFG Proteintech Cat# 11571-1-AP; RRID: AB_2203102 WB (1:2000) IHC (1:200) Mouse anti-β-actin Proteintech Cat# 20536-1-AP; RRID: AB_10700003 WB (1:5000) Rabbit anti-GAPDH Proteintech Cat# 10494-1-AP; RRID: AB_2263076 WB (1:5000) Rabbit anti-vimentin Proteintech Cat# 10366-1-AP; RRID: AB_2273020 WB (1:5000) Rabbit anti-LIPC Proteintech Cat# 21133-1-AP; RRID: AB_11041715 IHC (1:200) Rabbit anti-TP53 Proteintech Cat# 10442-1-AP; RRID: AB_2206609 IHC (1:300) Rabbit anti-SKP1 Proteintech Cat# 10990-2-AP; RRID: AB_2187492 WB (1:1000) Rabbit anti-PAM Proteintech Cat# 26972-1-AP; RRID: AB_2880706 WB (1:3000) Rabbit anti-ATF2 Proteintech Cat# 14834-1-AP; RRID: AB_10694139 WB (1:1000) Goat anti-mouse IgG HRP Cell Signaling Cat# 7076S; RRID: AB_330924 WB (1:5000) Goat anti-rabbit IgG HRP Cell Signaling Cat# 7074S; RRID: AB_2099233 WB (1:5000) Goat anti-rat IgG HRP Cell Signaling Cat# 7077S; RRID: AB_10694715 WB (1:5000) Mouse anti-N-cadherin Cell Signaling Cat# 14215S; RRID: AB_2798427 WB (1:1000) Mouse anti-E-cadherin Cell Signaling Cat# 14472S; RRID: AB_2728770 WB (1:1000) Rabbit anti-snail Cell Signaling Cat# 3879S; RRID: AB_2255011 WB (1:1000) Rabbit anti-NF-κB p65 Cell Signaling Cat# 8242S; RRID: AB_10859369 WB (1:1000) Mouse IgG1 Isotype Control Cell Signaling Cat# 5415S; RRID: AB_10829607 Rabbit IgG1 Isotype Control Cell Signaling Cat# 3900S; RRID: AB_1550038
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